Lactobacillus delbrueckiisubsp.bulgaricusProteinase: Purification by Ion-Exchange and Hydrophobic Interaction Chromatography
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چکیده
منابع مشابه
Purification of bradykinin by ion-exchange chromatography.
Shirai, Y. & Ohkubo, T. (1954a). J. Biochem., Tokyo, 41, 337. Shirai, Y. & Ohkubo, T. (1954b). J. Biochem., Tokyo, 41, 341. Storey, I. D. E. (1950). Biochem. J. 47, 212. Storey, I. D. E. & Dutton, G. J. (1955). Biochem. J. 59,279. Storey, I. D. E. & Dutton, G. J. (1956). Proc. 3rd Int. Congr. Biochem., Brussel8, 1955, p. 162. Strominger, J. L., Kalckar, H. M., Axelrod, J. & Maxwell, E. S. (1954...
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ژورنال
عنوان ژورنال: International Journal of Food Properties
سال: 2014
ISSN: 1094-2912,1532-2386
DOI: 10.1080/10942912.2014.921199